Low-CpG bsr

Synthetic blasticidin resistance gene in cloning plasmid

Resistance to the antibiotic Blasticidin S is conferred by the bsr gene from Bacillus cereus.

The DNA sequence of this gene was modified by optimizing the codon usage and reducing the number of CpG motifs from 14 to 4 without changing the amino acid sequence of the wild type protein.

The low-CpG Bsr gene is provided in the bacterial plasmid pMOD2 which is selectable with Ampicillin.

To facilitate the excision and subcloning of the low-CpG Bsr gene into another vector, this gene is flanked by a unique restriction site at the 5’ and 3’ end.

Specifications

Gene: Low-CpG Bsr

Description: Synthetic Blasticidin resistance gene

Backbone: pMOD2

Selection: Ampicillin

14 CpG in the native gene, 4 CpG in the synthetic gene

Contents

• 20 µg of lyophilized DNA

Product is shipped at room temperature.

Lyophilized DNA should be resuspended upon receipt and stored at -20°C.

Description

PLASMID FEATURES

• Multiple cloning sites
MCS1, located upstream of the synthetic gene, contains the following restriction sites:
Nde I, Bst EII, Avr II, Mfe I, Bgl II, Hind III, Pme I, Bsp HI
MCS2, located downstream of the synthetic gene, contains the following restriction sites:
Eco RI, Bam HI, Nhe I, Eco RV, Sac I, Pac I
• EM7: This bacterial promoter enables the constitutive expression of the antibiotic resistance gene in E. coli.
• Synthetic Bsr gene (bsr-lowCpG): The bsr gene from Bacillus cereus is a small gene (420 bp) that encodes a deaminase and confers resistance to the antibiotic Blasticidin S. The bsr gene was modified by optimizing the codon usage and reducing the number of CpG motifs from 14 to 4.
• rnpb term: The E. coli rnpb terminator allows efficient transcription termination of the bsr-lowCpG gene.
• pMB1 Ori: To limit vector size, InvivoGen uses a minimal E. coli origin of replication with the same activity as the longer Ori.
• Amp: The ampicillin resistance gene allows the selection of transformed E. coli carrying a pMOD plasmid.